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Substrates

 

 

 

 
Substrates of Erk-1,2 = p42, p44 MAP kinases
Substrates Accession No Sites Reference
AML1, PEBP2 alpha isoform B,
runt related transcription factor		 Q01196 Ser249, Ser266 Tanaka et al., 1996 MCB 16:3967-79
Amphiphysin 1 P49418 Ser285, Ser293 Shang et al., 2004 JBC 279:40890-6
BCL2 P10415 Ser87 Tamura et al., 2004 FEBS Lett. 569:249-55
BIM (Bcl2 like 11 isoform 1) NP_619527 Ser59, Ser69, Ser104 Harada et al., 2004 PNAS USA 101:15313-7
BRF   Thr145 and other Felton-Edkins et al., 2003 EMBO J. 22:2422-2432
CD120a (TNF-R1 p55) P19438 (mouse) Thr276, Thr280, Ser286 Cottin et al., 1999 JBC 274:32975-32987
Crystallin alpha B
heat shock protein beta 5		 NP_001876 Ser59 Kato et al., 1998 JBC 273:28346-54
mCRY1 chryptochrome NP_031797 (mouse) Ser247 Sanada et al., 2004 Genes Dev. 9:697-708
mCRY2 chryptochrome NP_034093 (mouse) Ser265, Ser557 Sanada et al., 2004 Genes Dev. 9:697-708
ENaC epithelial Na+ channel beta NP_000327 Thr615 or Thr613 in mouse Shi et al., 2002 JBC 277:13539-47
ENaC gamma NP_001030 Thr622 Shi et al., 2002 JBC 277:13539-47
ETS1transcription factor   Thr38

Rabault et al., 1996 Oncogene 13:877-881

ETS translocation variant 1, ER81 P50549 probably Ser94, Thr138, Ser146 Jahnknecht 1996 MCB 16:1550-6
ELK1transcription factor NP_005220   Cano et al., 1995 J. Cell Sci. 108:3599-3609
ELK3, NET, SAP-2 transcription factor P41970 C-domain Hill CS and Treisman R. 1995 Cell 80:199-211
GAB1 Grb2-associated binder 1 AAH64848 Thr476 Roshan et al., 1999 JBC 274:36362-36368
GAB2 Grb2 associated binder 2 Q9UQC2 Ser623 Arnaud et al., 2004 J. Immunol. 173:3962-71
GATA-1 transcription factor P17679 (mouse) Ser26, Ser178 Towatari et al., 2004 Hematol . J. 5:262-72
GRASP55 Golgi reassembly stacking protein 55 NP_056345 Thr222, Thr225 Jesch et al., 2001 Mol. Biol. Cell 12:1811-7
hnRNP-K heterogeneous nuclear ribonucleoprotein K NP_002131 Ser284, Ser353 Habelnah et al., 2001 Nat. Cell. Biol. 3:325-30
HSL hormone sensitive lipase   Ser600 Greenberg et al., 2001 JBC 276:45456-61
HSF1 (heat shock factor 1, heat shock transcription factor 1; HSTF1) Q00613 Ser 303 and Ser 307

Dai et al., 2000 JBC 275:18210-8
HIV1alpha gag-MA protein     Jacque et al., 1998 EMBO J. 17:2607-2618
IEX-1 NP_434702 Thr18 Garcia et al., 2004 EMBO J. 21:5151-63
KV4.2 NP_036413 Thr602, Thr607, Ser616 Adams et al., 2000 J Neurochem 75:2277-2287
Krueppel like factor 11, KLF11, TIEG-2 O14901   Ellenrieder et al., 2004 Gastroenterology 127:607-20
KSR1 NP_038599 (mouse) Thr260, Thr274, Ser443 Cacace et al., 1999 MCB 19:229-240
MAP2K1, MEK1, MKK1 NP_002746 Thr292 Eblen et al., 2004 MCB 24:2308-17
MBP myelin basic protein     Commonly used in vitro substrate
MNK1 Q9BUB5 Thr221?, Thr226?, Thr385? Waskiewicz et al., 1997 EMBO J. 16:1909-20
MNK2 Q8CDB0 Thr 197, Thr 202 and Thr 332 Waskiewicz et al., 1997 EMBO J. 16:1909-20
MSK1, MSK2 AAC31171 Thr 581 Deak et al., 1998 EMBO J. 17;:4426-41
Na+,K+ ATPase alpha(1)-subunit   Ser11 Khundmiri et al., 2004 JBC 279:17418-27
NFATc NP_775114 Ser-Pro repeats Porter et al., 2000 JBC 275:3543-51
Paxillin NP_035353 (mouse) Ser83 Ishibe et al., Mol. Cell 16:257-67
PDE4D NP_006194 Ser579 Hoffmann et al., 1999 EMBO J. 18:893-903

p90 ribosomal kinases, RSK1, 2, 3

     Zhao et al., 1996 JBC 271:29773-9
RNA Polymerase II P24928 C-terminus Markowitz et al., 1995 BBRC 207:1051-7
p27KIP1, CDK inhibitor 1B NP_004055 Ser178 Wolf et al., 2003 Diabetologia 46:1090-9
RSK2 P51812 Ser369, Thr577  
SOS NP_005624 several c-terminal Ser-Pro Chemiack et al., 2994 JBC 269:4717-20
SP1 NP_612482   Merchant et al., 1999 BBRC 254:454-61
SAP90 (synapse-associated protein 90, PSD-95 Q62108 Thr287 and Ser290 Sabio et al., 2004 Biochem J. 380: 19-30

SREBP-2
sterol regulatory element binding protein 2

NP_004590 Ser432, Ser455 Kotzka et al., 2004 JBC 279:22404-11
TAU   hyperphosphorylation Blanchard et al., 1994 BBRC 200:187-94
TNF alpha converting enzyme, TACE, ADAM 17 P78536 Thr735 Diaz-Rodriguez et al., Mol. Biol. Cell 13:2031-44
Tyrosine hydroxylase NP_000351 Ser31 Sutherland et al., 1993 Eur. J. Biochem. 217:715-22
Vinexin beta, SH3 domain protein 4 NP_035496 (mouse) Ser 191 Mitsushima et al., 2004 JBC:279:34570-7

 
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