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Substrates

 

 

 

Substrates of PKA, cAMP activated protein kinase
Substrates Acc. No Sites Reference
CREB P16220 Ser133 Fiol et al.,1987 JBC 262 14042-48
class C L-type Ca(2+) channel (Ca(v)1.2) Q01815 Ser 1928 Davare et al., 2000 JBC 275:39710-7.
Engrailed-2 NP_001418 Ser276 Hjerrilde et al., 2004 FEBS Lett. 568:55-59
GRK1 (GPCR kinase 1) = Rhodopsin kinase Q15835 Ser21 Horner et al., 2005 in press
GRK7 Q8WTQ7 Ser23, Ser36 Horner et al., 2005 in press
HePTP (haematopoietic protein tyrosine phosphatase) P35236 Ser44 Saxena et al., 1999 Nat Cell Biol 1:305-11
Hormone sensitive lipase Q05469 Ser 565 Donsmark et al., 2004 Biochem Biophys Res Commun. 316:867-71
Inhibitor of Protein Phosphatase 1 (IPP-1) Q13522 Thr35 Endo et al., 1996 Biochemistry 35:5220-5228
Intermediate conductance calcium-activated potassium channel protein 1 (putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel) NP_002241 Ser 312, Thr 327, Ser 332, Thr 348 Gerlach et al., 2000 JBC. 275:585-98
Lactase-phlorizin hydrolase preproprotein (lactase-phlorizin hydrolase, lactase-glycosylceramidase) NP_002290 Ser 1916 Keller et al., 1995 FEBS Lett. 368:563-7
5-Lipoxygenase P09917 Ser523 Luo et al., 2004 JBC in press

Moesin-ezrin-radizin-like protein (MERLIN, Neurofibromin 2, schwannomerlin)

NP_861969 Ser 518 Alfthan et al., 2004 JBC 279:18559-66.
Myosin-binding protein C, Q14896 Ser 265, Ser 300, Thr 274 Mohamed et al., 1998
Arch Biochem Biophys. 358:313-9
MYPT1 O14974 Ser695 Woolridge et al., 2004 JBC in press
NIPP-1 (Nuclear inhibitor of protein phosphatase1) Q12972 Ser178, Ser199 Vulsteke et al., 1997 JBC 272:32972-32978
Rap-1 small GTP binding protein P62834 Ser180 Lerosey et al., 1991 BBRC 175:430-436
RIM1 alpha Q9JIR4 (rat) Ser413, Ser1548 ? Lonart et al., 2003 Cell 115:49-60
RelA (NF-kappa-B p65 subunit) Q04206 Ser276 Zhong et al., 1997 Cell 89:413-424
RhoA (ras homolog gene family, member A; Rho family GTPase) NP_058082 Ser 188 Dong et al., 1998 JBC 273:22554-62.
Transition Protein 2 (TP2) NP_058753 (rat) Thr101, Ser109 Meetei et al., 2002 Biochemistry 41:185-95
VASP (vasodilator-stimulated phosphoprotein) NP_003361 Ser 157, Ser 239, Thr 278 Li et al., 2003 Blood. 101:4423-9
Tau AAC04279 Ser 262, Ser320, Ser324, Ser356 Schneider et al., 1999 Biochemistry 38:3549-58

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axel.knebel@kinasource.co.uk